Curation Information

CcpA-mediated repression of streptolysin S expression and virulence in the group A streptococcus.;Kinkel TL, McIver KS;Infection and immunity 2008 Aug; 76(8):3451-63 [18490461]
CcpA [A0A0C6FYN7, view regulon]
Reported TF sp.
Streptococcus pyogenes MGAS5005
Reported site sp.
Streptococcus pyogenes MGAS5005
Created by
Matthew Coveyou
Curation notes

Experimental Process

DNA-array expression analysis was first used to identify variation in gene expression between a wild type and ccpA-mutant strain. qRT-PCR confirmed these results for twelve genes, including sagA/pel. In silico analysis previously identified a catabolite response element (CRE), and luciferase assays confirmed sagA was regulated by CCR. EMSA demonstrated specific binding of ccpA to the CRE, and showed Hpr-P-Ser did not improve binding to this site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... sagA
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified