DNA-array expression analysis was first used to identify variation in gene expression between a wild type and ccpA-mutant strain. qRT-PCR confirmed these results for twelve genes, including sagA/pel. In silico analysis previously identified a catabolite response element (CRE), and luciferase assays confirmed sagA was regulated by CCR. EMSA demonstrated specific binding of ccpA to the CRE, and showed Hpr-P-Ser did not improve binding to this site.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|