Curation Information

The gdhB gene of Pseudomonas aeruginosa encodes an arginine-inducible NAD(+)-dependent glutamate dehydrogenase which is subject to allosteric regulation.;Lu CD, Abdelal AT;Journal of bacteriology 2001 Jan; 183(2):490-9 [11133942]
ArgR [G3XCU2, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Grace Chandler
Curation notes

Experimental Process

Visual sequence inspection upstream of gdhB revealed a putative ArgR binding site based on similarity to the ArgR consensus. A ghdB::lacZ transcriptional fusion assay using an argR knockout strain demonstrated the role of ArgR as an activator of arginine dependent induction. Multiple sequence alignment deduced an ArgR consensus sequence.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... gdhB PA3067 PA3066 PA3065
Experimental technique details Beta-gal reporter assay - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details Visual sequence inspection (nan) - activator not specified