Visual sequence inspection upstream of gdhB revealed a putative ArgR binding site based on similarity to the ArgR consensus. A ghdB::lacZ transcriptional fusion assay using an argR knockout strain demonstrated the role of ArgR as an activator of arginine dependent induction. Multiple sequence alignment deduced an ArgR consensus sequence.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|