Curation Information

Regulation of the dauBAR operon and characterization of D-amino acid dehydrogenase DauA in arginine and lysine catabolism of Pseudomonas aeruginosa PAO1.;Li C, Yao X, Lu CD;Microbiology (Reading, England) 2010 Jan; 156(Pt 1):60-71 [19850617]
ArgR [G3XCU2, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Grace Chandler
Curation notes

Experimental Process

A dauB::lacZ fusion assay using an argR dauR mutant identified ArgR-dependent activation of the dauBAR operon. Sequence analysis of the dauB region revealed a potential ArgR binding site. EMSA using purified ArgR and the regulatory region of dauB demonstrated ArgR binding. Site-directed mutagenesis was used to introduce mutations to the putative ArgR binding site. EMSA performed with the mutated dauBAR promoter showed that ArgR binding to this promoter was abolished.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... dauB dauA dauR
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified