Curation Information

Amino acid-mediated induction of the basic amino acid-specific outer membrane porin OprD from Pseudomonas aeruginosa.;Ochs MM, Lu CD, Hancock RE, Abdelal AT;Journal of bacteriology 1999 Sep; 181(17):5426-32 [10464217]
ArgR [G3XCU2, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Grace Chandler
Curation notes

Experimental Process

Consensus search upstream of the oprD start codon revealed a putative binding sequence. EMSA of the oprD upstream region demonstrated AlgR binding to this region. DNase I footprinting revealed that ArgR protected a 47 bp region containing the predicted binding site. RNA dot blot identified that ArgR activated the expression of oprD in response to exogenous arginine. Multiple sequence alignment was used to derive a consensus binding sequence for ArgR.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... oprD PA0959 PA0960
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details RNA dot blot (ECO:0001177) - activator not specified