Curation Information

Regulation of the expression of the CmeABC efflux pump in Campylobacter jejuni: identification of a point mutation abolishing the binding of the CmeR repressor in an in vitro-selected multidrug-resistant mutant.;Cagliero C, Maurel MC, Cloeckaert A, Payot S;FEMS microbiology letters 2007 Feb; 267(1):89-94 [17166222]
CmeR [Q0PBE2, view regulon]
Reported TF sp.
Campylobacter jejuni 154KU
Reported site sp.
Campylobacter jejuni 154KU
Created by
Dinara Sagitova
Curation notes

Experimental Process

Transcriptional lacZ fusions of the cmeABC-promoter region showed that mutated cmeABC-lacZ had a fourfold higher activity then the wild-type promoter. EMSA confirmed binding of CmeR with the cmeR-cmeA intergenic sequence.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... cmeA cmeB cmeC
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - repressor dimer