Transcriptional lacZ fusions of the cmeABC-promoter region showed that mutated cmeABC-lacZ had a fourfold higher activity then the wild-type promoter. EMSA confirmed binding of CmeR with the cmeR-cmeA intergenic sequence.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|TGTAATAAAAATTACA||cmeA, cmeB, cmeC||