Curation Information

CmeR functions as a transcriptional repressor for the multidrug efflux pump CmeABC in Campylobacter jejuni.;Lin J, Akiba M, Sahin O, Zhang Q;Antimicrobial agents and chemotherapy 2005 Mar; 49(3):1067-75 [15728904]
CmeR [Q0PBE2, view regulon]
Reported TF sp.
Campylobacter jejuni subsp. jejuni 81-176
Reported site sp.
Campylobacter jejuni subsp. jejuni 81-176
Created by
Dinara Sagitova
Curation notes

Experimental Process

Immunoblotting with CmeABC-specific antibodies showed that expression of cmeB was about fivefold higher in cmeR mutant strain than in the wild-type strain. Additionally, cmeABC-lacZ reporter assay showed that expression of cmeB was approximately 6.2fold higher in cmeB mutant than in the wild-type indicating that CmeR represses the transcription of cmeABC. EMSA confirmed that CmeR regulates the cmeABC operon via direct interaction with the promoter of cmeABC. EMSAs performed with a series of PCR products whose sequences span different portions of the IT region between cmeR and cmeABC confirmed specific CmeR binding. Single nucleotide deletion in conjunction with cmeABC-lacZ reporter assay further confirmed specific binding of CmeR to its target promoter.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type