Curation Information

Publication

CmeR functions as a transcriptional repressor for the multidrug efflux pump CmeABC in Campylobacter jejuni.;Lin J, Akiba M, Sahin O, Zhang Q;Antimicrobial agents and chemotherapy 2005 Mar; 49(3):1067-75 [15728904]

TF

CmeR [Q0PBE2, view regulon]

Reported TF sp.

Campylobacter jejuni subsp. jejuni 81-176

Reported site sp.

Campylobacter jejuni subsp. jejuni 81-176

Created by

Dinara Sagitova

Curation notes

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Experimental Process

Immunoblotting with CmeABC-specific antibodies showed that expression of cmeB was about fivefold higher in cmeR mutant strain than in the wild-type strain. Additionally, cmeABC-lacZ reporter assay showed that expression of cmeB was approximately 6.2fold higher in cmeB mutant than in the wild-type indicating that CmeR represses the transcription of cmeABC. EMSA confirmed that CmeR regulates the cmeABC operon via direct interaction with the promoter of cmeABC. EMSAs performed with a series of PCR products whose sequences span different portions of the IT region between cmeR and cmeABC confirmed specific CmeR binding. Single nucleotide deletion in conjunction with cmeABC-lacZ reporter assay further confirmed specific binding of CmeR to its target promoter.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.