Experimenters first identified 2 putative binding sites in the flhDC promoter region. Next they ran an EMSA of the promoter region, and found that OmpR bound it. Finally, they used a OmpR::lacZYA operon fusion to preform a B-gal assay which showed a positive regulatory role for OmpR
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|