Curation Information

Publication
Promoter specificity in Pseudomonas aeruginosa quorum sensing revealed by DNA binding of purified LasR.;Schuster M, Urbanowski ML, Greenberg EP;Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 9; 101(45):15833-9 [15505212]
TF
LasR [P25084, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

EMSA showed that LasR bound specifically to the las-rhl box in the lasI-rsaL intergenic region. DNase I footprinting showed that LasR protected a region that included the 20-bp las-rhl box.

Transcription Factor Binding Sites


ATCTATCTCATTTGCTAGTT
ATCTATCTCATTTGCTAGTT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ATCTATCTCATTTGCTAGTT
... ... rsaL lasR lasI PA1433 PA1434
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - activator dimer