RT-PCR analysis showed that OmpR represses invasin expression at 25C. inv-lacZ reporter gene fusion confirmed that OmpR negatively regulates inv promoter. EMSAs were done to confirm specific binding of OmpR to its target binding site on the inv promoter.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|