Curation Information

Genome-wide survey for PilR recognition sites of the metal-reducing prokaryote Geobacter sulfurreducens.;Krushkal J, Juárez K, Barbe JF, Qu Y, Andrade A, Puljic M, Adkins RM, Lovley DR, Ueki T;Gene 2010 Dec 1; 469(1-2):31-44 [20708667]
PilR [Q74D24, view regulon]
Reported TF sp.
Geobacter sulfurreducens PCA
Reported site sp.
Geobacter sulfurreducens PCA
Created by
Erill Lab
Curation notes

Experimental Process

Computational search used to predict PilR sites genome-wide using previous known consensus from Pseudomonas. Site upstream of pilA shown to be functional by luciferase assays with constructs containing or not the site, but maintaining the RpoN site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... pilA-N pilR pilS pilC pilT-4 pilB aroE GSU1489 GSU1488 ribF tatC rnr pilA-C xapA xapB GSU1500 xapD xapE xapF xapG xapH xapI xapJ GSU1508 GSU1509 GSU1510 GSU1510.1 GSU1511 GSU1512 GSU1513 GSU1514 GSUR026 thrS infC rpmI rplT pheS pheT ihfA-1 GSU1522 GSUR027 surE pcm rpoS apt GSUR028
Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details Regular expression search (ECO:0005662) - activator not specified