Curation Information

LexA regulates the bidirectional hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803 as a transcription activator.;Gutekunst K, Phunpruch S, Schwarz C, Schuchardt S, Schulz-Friedrich R, Appel J;Molecular microbiology 2005 Nov; 58(3):810-23 [16238629]
LexA [P73722, view regulon]
Reported TF sp.
Synechocystis sp. PCC 6803
Reported site sp.
Synechocystis sp. PCC 6803
Created by
Erill Lab
Curation notes

Experimental Process

Binding of LexA first identified with EMSA on crude extract plus 2D-PAGE and MALDI-TOF, then validated with purified LexA and a single oligo for EMSA. Luciferase assay with overlapping oligo indicates that high expression of the gene is dependent on the same region containing the putative LexA-binding site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... MYO_115360 MYO_115370 hoxH MYO_115300 MYO_115310 hoxY hoxU MYO_115340 hoxF
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details MALDI-TOF Mass Spectrometry (ECO:0005527) - activator dimer