The PSSM was used to predict the presence of Fur box-like sites within the hmsT promoter region. Fur-mediated transcription regulation was confirmed by conducting primer extension and beta-gal reporter assays. EMSA showed that Fur bound its target sites. DNase I footprinting assay was used to locate Fur-binding sites within the hmsT promoter region.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|