qRT-PCR was used to show Zur mediated repression of znu, zrf, and zra genes. Beta-gal fusion assays further confirmed Zn-dependent induction of the zrf and zra expression. EMSA confirmed Zur specific binding to the promoters of these genes. DNase I footprinting localized these Zur binding sites and further confirmed binding Visual sequence inspection and multiple sequence alignment identified a putative Zur consensus sequence.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|