Western blot (quantitative) expression analysis
A Western blot (also frequently called immunoblot) is a gel-based technique used to determine the presence of specific proteins in a sample by means of separation (through gel electrophoresis) and detection (using a protein-specific antibody). Less frequent than the equivalent RNA Northern blot, it is typically used in TF-binding site analysis to validate differential regulation of target genes.
Regulated genes for each binding site are displayed below. Gene regulation diagrams
show binding sites,
both positively and negatively regulated
genes, genes with unspecified type of regulation.
For each indvidual site, experimental techniques used to determine the site are also given.