Curation Information

Publication

The hdhA gene encodes a haloacid dehalogenase that is regulated by the LysR-type regulator, HdhR, in Sinorhizobium meliloti.;Sallabhan R, Kerdwong J, Dubbs JM, Somsongkul K, Whangsuk W, Piewtongon P, Mongkolsuk S, Loprasert S;Molecular biotechnology 2013 Jun; 54(2):148-57 [22638965]

TF

HdhR [Q92Y01, view regulon]

Reported TF sp.

Sinorhizobium meliloti 1021

Reported site sp.

Sinorhizobium meliloti 1021

Created by

Dinara Sagitova

Curation notes

The authors report that the HdhR protected region contains four LysR-type consensus sites (5'T–N11–A 3'). Individual sites were not listed.

Experimental Process

HdhR-mediated repression of hdhA was confirmed by lacZ promoter fusion and RT-PCR assays which showed that hdhA expression increased significantly in the hdhR mutant compared to the wild-type S. meliloti 1021 strain. EMSA verified that HdhR binds to the hdhA promoter fragment from +80 to -118 bp relative to the hdhA translation start. DNase I footprinting analyses determined that HdhR protected two regions from approximately -12 to -37 and from -42 to -67 bps upstream of the hdhA start codon.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.