Curation Information

Publication

Direct transcriptional control of the plasminogen activator gene of Yersinia pestis by the cyclic AMP receptor protein.;Kim TJ, Chauhan S, Motin VL, Goh EB, Igo MM, Young GM;Journal of bacteriology 2007 Dec; 189(24):8890-900 [17933899]

TF

CRP [Q7CFV3, view regulon]

Reported TF sp.

Yersinia pestis GYS475

Reported site sp.

Yersinia pestis GYS475

Created by

Grace Chandler

Curation notes

The reported binding site did not match the genome. Possibly a bad proxy strain.

Experimental Process

A pla-lacZ fuison determined CRP activates pla expression. Visual inspection of the pla upstream region revealed a sequence similar to the CRP binding motif in E. coli. EMSA confirmed binding to the pla upstream region. DNase I footprinting localized this binding site. Site-directed mutagenesis in conjunction with pla-lacZ fusion assays demonstrated the specificity of CRP binding.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.