Curation Information

Publication
Multiple small RNAs act additively to integrate sensory information and control quorum sensing in Vibrio harveyi.;Tu KC, Bassler BL;Genes & development 2007 Jan 15; 21(2):221-33 [17234887]
TF
LuxO [A7MVC2, view regulon]
Reported TF sp.
Vibrio harveyi BB120
Reported site sp.
Vibrio harveyi BB120
Created by
Erill Lab
Curation notes
-

Experimental Process

Binding sites were identified by similarity to known consensus. RT-PCR measurements on a constitutively active LuxO mutant show differential overexpression of qrr genes. The downstream effect of its regulation on qrgA was observed phenotypically.

Transcription Factor Binding Sites


TTGCATTTTGCAA
TTGCATAATGCAA
TTGCAATTTGCAA
TTGCATTTTGCAA
TTGCAAAATGCGT
TTGCGATTTGCAA
TTGCAATATGCAT
TTGCATTTTGCAA
TTGCATAATGCAA
TTGCAATTTGCAA
TTGCATTTTGCAA
TTGCAAAATGCGT
TTGCGATTTGCAA
TTGCAATATGCAT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TTGCATTTTGCAA VIBHAR_06697,
... ... VIBHAR_06697 VIBHAR_06698
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified
TTGCATAATGCAA VIBHAR_04846
... ... VIBHAR_04846
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified
TTGCAATTTGCAA VIBHAR_05322
... ... VIBHAR_05322
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified
TTGCATTTTGCAA VIBHAR_06697,
... ... VIBHAR_06697 VIBHAR_06698
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified
TTGCAAAATGCGT VIBHAR_06697,
... ... VIBHAR_06697 VIBHAR_06698
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified
TTGCGATTTGCAA VIBHAR_05886,
... ... VIBHAR_05886 VIBHAR_05885 VIBHAR_05884 VIBHAR_05883 VIBHAR_05882
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified
TTGCAATATGCAT VIBHAR_05886,
... ... VIBHAR_05886 VIBHAR_05885 VIBHAR_05884 VIBHAR_05883 VIBHAR_05882
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified