Binding sites were identified by similarity to known consensus. RT-PCR measurements on a constitutively active LuxO mutant show differential overexpression of qrr genes. The downstream effect of its regulation on qrgA was observed phenotypically.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
Site sequence | Regulated genes | Gene diagram | Experimental techniques | TF function | TF type |
---|---|---|---|---|---|
TTGCATTTTGCAA | VIBHAR_06697, |
|
activator | not specified | |
TTGCATAATGCAA | VIBHAR_04846 |
|
activator | not specified | |
TTGCAATTTGCAA | VIBHAR_05322 |
|
activator | not specified | |
TTGCATTTTGCAA | VIBHAR_06697, |
|
activator | not specified | |
TTGCAAAATGCGT | VIBHAR_06697, |
|
activator | not specified | |
TTGCGATTTGCAA | VIBHAR_05886, |
|
activator | not specified | |
TTGCAATATGCAT | VIBHAR_05886, |
|
activator | not specified |