Curation Information

Publication

Reversible acyl-homoserine lactone binding to purified Vibrio fischeri LuxR protein.;Urbanowski ML, Lostroh CP, Greenberg EP;Journal of bacteriology 2004 Feb; 186(3):631-7 [14729687]

TF

LuxR [B5EV73, view regulon]

Reported TF sp.

Vibrio Fischeri MJ-1

Reported site sp.

Vibrio Fischeri MJ-1

Created by

Dinara Sagitova

Curation notes

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Experimental Process

DNase I footprinting showed that LuxR protected a 28bp region which contained a 20-bp lux box. In vitro transcription assay showed that LuxR activated luxI transcription.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.