Curation Information

Publication

Characterization of cis-acting sites controlling arginine deiminase gene expression in Streptococcus gordonii.;Zeng L, Dong Y, Burne RA;Journal of bacteriology 2006 Feb; 188(3):941-9 [16428398]

TF

ArcR [A0A0F3HTU8, view regulon]

Reported TF sp.

Streptococcus gordonii DL1

Reported site sp.

Streptococcus gordonii DL1

Created by

Matthew Coveyou

Curation notes

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Experimental Process

EMSA of arcR with an upstream region of the arc promoter demonstrated specific binding, improved by arginine. Additional site-deleted probes indicated only one binding site, but low-level residual binding suggested one or more low-affinity bindings sites as well. DNase I protection was used to identify the binding site. CAT assays with glucose and galactose indicated the arc operon is subject to CCR, and partial-deletion mutant strains of the binding site demonstrated the entire protected region to be necessary for binding.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.