Curation Information

Publication

Genetic characterization of the CcpA-dependent, cellobiose-specific PTS system comprising CelB, PtcB and PtcA that transports lactose in Lactococcus lactis IL1403.;Aleksandrzak-Piekarczyk T, Polak J, Jezierska B, Renault P, Bardowski J;International journal of food microbiology 2011 Jan 31; 145(1):186-94 [21262549]

TF

CcpA [Q9CF33, view regulon]

Reported TF sp.

Lactococcus lactis subsp. lactis Il1403

Reported site sp.

Lactococcus lactis subsp. lactis Il1403

Created by

Dinara Sagitova

Curation notes

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Experimental Process

Visual inspection of the promoter regions of the ybhE, bglS, and ptcB genes identified CcpA binding sequences with less than 2 mismatches with the CcpA consensus. Promoter luciferase reporter assays performed in the wild type and ccpA mutant strains identified that in the absence of CcpA repression of these genes was relieved.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.