Curation Information

Publication

Involvement of regulatory interactions among global regulators GlxR, SugR, and RamA in expression of ramA in Corynebacterium glutamicum.;Toyoda K, Teramoto H, Gunji W, Inui M, Yukawa H;Journal of bacteriology 2013 Apr; 195(8):1718-26 [23396909]

TF

GlxR [Q79VI7, view regulon]

Reported TF sp.

Corynebacterium glutamicum R

Reported site sp.

Corynebacterium glutamicum R

Created by

Joe Sparenberg

Curation notes

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Experimental Process

EMSA and site directed mutagenesis confirmed the sole binding site of GlxR in the ramA promoter that was identified previously by chromatin immunoprecipitation analysis. Site directed mutagenesis and lacZ analysis showed that GlxR activates ramA expression.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.