Curation Information

Publication

The Type II Secretion Pathway in Vibrio cholerae Is Characterized by Growth Phase-Dependent Expression of Exoprotein Genes and Is Positively Regulated by σE.;Zielke RA, Simmons RS, Park BR, Nonogaki M, Emerson S, Sikora AE;Infection and immunity 2014 Jul; 82(7):2788-2801 [24733097]

TF

RpoE [Q9KPA6, view regulon]

Reported TF sp.

Vibrio cholerae O395

Reported site sp.

Vibrio cholerae O395

Created by

Dinara Sagitova

Curation notes

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Experimental Process

A putative sigma-E binding site that had one mismatch with the V.cholerae and E.coli sigma-E consensus was identified in the eps regulatory region. rpoEP2-lux promoter fusion showed that s iron starvation and exposure to polymyxin B resulted in 4- and 6-fold increases, respectively, in transcriptional activity. esp-lux fusion with an esp promoter that lacked a putative sigma-E binding site showed that RpoE had no effect on the expression compared to the wild type promoter fusion in which overexpression of RpoE resulted in over 4-fold induction of the esp expression. The immunoblotting analyses showed that RpoE positively regulated the expression of T2S at the protein level. Peps-lux fusion assays in wild type O395 and the rpoE mutant strains showed that there was a statistically significant 2-fold induction of eps-lux activity in the wild-type V. cholerae.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.