Curation Information

Publication

Interaction of two LysR-type regulatory proteins CatR and ClcR with heterologous promoters: functional and evolutionary implications.;Parsek MR, McFall SM, Shinabarger DL, Chakrabarty AM;Proceedings of the National Academy of Sciences of the United States of America 1994 Dec 20; 91(26):12393-7 [7809047]

TF

ClcR [A0A024HKB0, view regulon]

Reported TF sp.

Pseudomonas putida plasmid pAC27

Reported site sp.

Pseudomonas putida plasmid pAC27

Created by

Matthew Coveyou

Curation notes

ClcR and clcABD are borne on the Pseudomonas putida plasmid pAC27, which has no RefSeq equivalent. Bordetella petrii DSM 12804 (NC_010170.1) has a clcR gene (YP_001630141) which has been used as a placeholder.

Experimental Process

DNase I protection of the plasmid-borne clcABD promoter and chromosomal catBC promoter generated a binding footprint for ClcR on each. EMSA confirmed binding to these regions. Qualitative growth analysis and beta-gal assay demonstrated that despite confirmed binding to the catBC promoter, clcR did not activate its transcription.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.