Curation Information

Publication

Critical nucleotides in the interaction of CatR with the pheBA promoter: conservation of the CatR-mediated regulation mechanisms between the pheBA and catBCA operons.;Tover A, Zernant J, Chugani SA, Chakrabarty AM, Kivisaar M;Microbiology (Reading, England) 2000 Jan; 146 ( Pt 1)():173-83 [10658664]

TF

CatR [Q88GK5, view regulon]

Reported TF sp.

Pseudomonas putida PaW85

Reported site sp.

Pseudomonas putida plasmid pAT1140

Created by

Matthew Coveyou

Curation notes

-

Experimental Process

CatR regulation of the pheBA operon in Pseudomonas putida was investigated as it related to an internal binding site (IBS), identified by DNase I protection in an earlier publication. Deletion analysis and site-directed mutagenesis demonstrated reduced pheBA expression so long as both the recognition binding site (RBS) and activation binding site (ABS) were intact.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.