Curation Information

Publication

Recruitment of sigma54-RNA polymerase to the Pu promoter of Pseudomonas putida through integration host factor-mediated positioning switch of alpha subunit carboxyl-terminal domain on an UP-like element.;Macchi R, Montesissa L, Murakami K, Ishihama A, De Lorenzo V, Bertoni G;The Journal of biological chemistry 2003 Jul 25; 278(30):27695-702 [12754257]

TF

IHF [P0A126, view regulon]

Reported TF sp.

Pseudomonas putida

Reported site sp.

Pseudomonas putida

Created by

Grace Chandler

Curation notes

The strain was not specified in the paper, making finding a proxy strain difficult. The genes did not match P. putida 2440. A proxy strain needs to be determined.

Experimental Process

EMSA demonstrated binding of IHF to the Pu promoter. DNase I footprinting identified the location of the IHF binding site. Site directed mutagenesis combined with EMSA confirmed the identity and specificity of this promoter sequence.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.