Curation Information

Publication

In vivo and in vitro evidence that TtgV is the specific regulator of the TtgGHI multidrug and solvent efflux pump of Pseudomonas putida.;Rojas A, Segura A, Guazzaroni ME, Terán W, Hurtado A, Gallegos MT, Ramos JL;Journal of bacteriology 2003 Aug; 185(16):4755-63 [12896994]

TF

TtgV [V9UYV3, view regulon]

Reported TF sp.

Pseudomonas putida DOT-T1E

Reported site sp.

Pseudomonas putida DOT-T1E

Created by

Dinara Sagitova

Curation notes

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Experimental Process

ttgG-lacZ and ttgV-lacZ reporter assays in the wild-type DOT-T1E and in the ttgV mutant strains identified that TtgV represses the expression of both ttgVW and ttgGHI operons. EMSA showed that TtgV bound to a 210-bp DNA fragment containing the sequence of the ttgV-ttgG intergenic region. Footprinting assays defined the region TtgV binding region within the ttgV-ttgG intergenic region.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome
• Site-specific quantitative values

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.