Curation Information

Publication

Purification and characterization of the Pseudomonas aeruginosa NfxB protein, the negative regulator of the nfxB gene.;Shiba T, Ishiguro K, Takemoto N, Koibuchi H, Sugimoto K;Journal of bacteriology 1995 Oct; 177(20):5872-7 [7592337]

TF

NfxB [P32265, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAO1

Reported site sp.

Pseudomonas aeruginosa PAO1

Created by

Dinara Sagitova

Curation notes

-

Experimental Process

nfxB transcription start site was determined by primer extension. EMSA confirmed that NfxB specifically binds to its own promoter region. DNase I footprinting assay identified that the region from -20 to +83 of nfxB was protected from DNase I digestion by NfxB. Negative autoregulation of nfxB was shown by performing nfxB-lacZ fusion assays.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.