Curation Information

Publication

The homogentisate pathway: a central catabolic pathway involved in the degradation of L-phenylalanine, L-tyrosine, and 3-hydroxyphenylacetate in Pseudomonas putida.;Arias-Barrau E, Olivera ER, Luengo JM, Fernández C, Galán B, García JL, Díaz E, Miñambres B;Journal of bacteriology 2004 Aug; 186(15):5062-77 [15262943]

TF

HmgR [Q88E47, view regulon]

Reported TF sp.

Pseudomonas putida KT2440

Reported site sp.

Pseudomonas putida KT2440

Created by

Joe Sparenberg

Curation notes

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Experimental Process

In vivo transcriptional analyses showed that HmgR is a repressor of the hmgABC operon. lacZ promoter fusion analysis further confirmed the results. EMSA confirmed HmgR binding to hmgR-hmgA intergenic region. DNase I footprinting was used to localize the HmgR binding region.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.