Curation Information

Publication

Characterization of interactions between the transcriptional repressor PhlF and its binding site at the phlA promoter in Pseudomonas fluorescens F113.;Abbas A, Morrissey JP, Marquez PC, Sheehan MM, Delany IR, O'Gara F;Journal of bacteriology 2002 Jun; 184(11):3008-16 [12003942]

TF

PhlF [G8QCB1, view regulon]

Reported TF sp.

Pseudomonas fluorescens F113

Reported site sp.

Pseudomonas fluorescens F113

Created by

Grace Chandler

Curation notes

-

Experimental Process

phlF-lacZ and phlA-lacZ fusion assays determined phlABCD repression by PhlF in the early logarithmic growth phase of P. fluorescens, with PHL inhibiting this repression in later growth stages. EMSA using DNA fragments of varied lengths confirmed PhlF binding to the phlA-phlF intergenic region. Copper-phenanthroline footprinting localized the binding site.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.