Curation Information

Publication
CcpA ensures optimal metabolic fitness of Streptococcus pneumoniae.;Carvalho SM, Kloosterman TG, Kuipers OP, Neves AR;PloS one 2011; 6(10):e26707 [22039538]
TF
CcpA [A0A0H2ZQ65, view regulon]
Reported TF sp.
Streptococcus pneumoniae D39
Reported site sp.
Streptococcus pneumoniae D39
Created by
Elliot White
Curation notes
-

Experimental Process

Researchers used a consensus search of the CRE box (CcpA binding site) in the pneumoniae genome to compile a list of genes with CRE boxes in the promoter. They then cross-referenced that list with the results (trimmed to only significant results) of a DNA-array that compared wt to a CcpA deletion.

Transcription Factor Binding Sites


ATATAATCGCTATCAA
AAGAATGCGTTTTTAT
TTGTAAACGGTTTCTA
GAGAAAGGGTTTACAT
TATTAAACGCTTCCAA
ATATAATCGCTATCAA
AAGAATGCGTTTTTAT
TTGTAAACGGTTTCTA
GAGAAAGGGTTTACAT
TATTAAACGCTTCCAA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ATATAATCGCTATCAA strH,
... ... strH SPD_0064
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor dimer
AAGAATGCGTTTTTAT celA,
... ... celA SPD_0276
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor dimer
TTGTAAACGGTTTCTA SPD_0286,
... ... SPD_0286 SPD_0287
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor dimer
GAGAAAGGGTTTACAT fba,
... ... fba vncS vncR
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor dimer
TATTAAACGCTTCCAA atpE,
... ... atpE SPD_1342 atpA atpH atpF atpB SPD_1343 atpG
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor dimer