Curation Information

Publication

The Pseudomonas aeruginosa exotoxin A regulatory gene, ptxS: evidence for negative autoregulation.;Swanson BL, Colmer JA, Hamood AN;Journal of bacteriology 1999 Aug; 181(16):4890-5 [10438759]

TF

PtxS [G3XD97, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAO1

Reported site sp.

Pseudomonas aeruginosa PAO1

Created by

Grace Chandler

Curation notes

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Experimental Process

EMSA determined P. aeruginosa contains a protein that specifically binds to the ptxS upstream region. A P. aeruginosa PAO1::ptxS mutant was constructed, identifying PtxS as the ptxS binding protein. EMSA using PtxS expressed from the T7 promoter in E.coli K38 demonstrated specific PtxS binding to the ptxS upstream region. A ptxS::lacZ fusion was utilized, showing beta gal activity four- to five times higher in the ptxS knockout mutant, confirming PtxS as an auto-regulator. DNase I footprinting isolated the specific sequence for PtxS binding.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.