Curation Information

Publication

Molecular characterization of MexL, the transcriptional repressor of the mexJK multidrug efflux operon in Pseudomonas aeruginosa.;Chuanchuen R, Gaynor JB, Karkhoff-Schweizer R, Schweizer HP;Antimicrobial agents and chemotherapy 2005 May; 49(5):1844-51 [15855505]

TF

MexL [Q9HXW2, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAO1

Reported site sp.

Pseudomonas aeruginosa PAO1

Created by

Grace Chandler

Curation notes

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Experimental Process

EMSAs were performed on the 94-bp mexL-mexJ intergenic region, demonstrating that MexL binds specifically. Visual inspection revealed two inverted GTATTT repeats on the mexL-mexJ intergenic region, separated by 16 bp. DNase I footprinting was performed to further map the operator sites, revealing a single protected region on both the mexL and mexJ coding strands, with the regions almost perfectly overlapping. LacZ fusion assays demonstrated auto-repression of mexL with expression levels lower in the presence of MexL.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.