Curation Information

Publication

Transcriptional control of the Bradyrhizobium japonicum irr gene requires repression by fur and Antirepression by Irr.;Hohle TH, O'Brian MR;The Journal of biological chemistry 2010 Aug 20; 285(34):26074-80 [20573962]

TF

Irr [H7C6K3, view regulon]

Reported TF sp.

Bradyrhizobium diazoefficiens USDA 110

Reported site sp.

Bradyrhizobium diazoefficiens USDA 110

Created by

Grace Chandler

Curation notes

Irr acts as an antirepressor of the irr expression.

Experimental Process

qRT-PCR was used to determine that the irr gene expression is regulated by both iron and manganese. DNAse I footprinting identified the Irr binding site within the -64 to -39 region of the Irr promoter.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.