Curation Information

Publication

The distal ExsA-binding site in Pseudomonas aeruginosa type III secretion system promoters is the primary determinant for promoter-specific properties.;Brutinel ED, King JM, Marsden AE, Yahr TL;Journal of bacteriology 2012 May; 194(10):2564-72 [22408165]

TF

ExsA [P26993, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAO1

Reported site sp.

Pseudomonas aeruginosa PAO1

Created by

Dinara Sagitova

Curation notes

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Experimental Process

Site-directed mutagenesis with lacZ fusion assays confirmed that GnC and TGnnA sequences in the exsC promoter are required for ExsA-mediated regulation. EMSA verified ExsA binding to its target promoter.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.