Curation Information

Publication

FleQ, the major flagellar gene regulator in Pseudomonas aeruginosa, binds to enhancer sites located either upstream or atypically downstream of the RpoN binding site.;Jyot J, Dasgupta N, Ramphal R;Journal of bacteriology 2002 Oct; 184(19):5251-60 [12218010]

TF

FleQ [G3XCV0, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAK

Reported site sp.

Pseudomonas aeruginosa PAK

Created by

Dinara Sagitova

Curation notes

Matching genome is still in progress. No RefSeq for Pseudomonas aeruginosa PAK is available.

Experimental Process

lacZ reporter assays and EMSA results indicated that FleQ and RpoN directly upregulate the expression of several flagellar genes. Specific locations of the FleQ binding sites were determined by DNase I footprinting. Functionality of the FleQ binding site in the flhA promoter region was confirmed by site-directed mutagenesis, EMSA, and lacZ reporter fusion assay.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.