Curation Information

Publication

Two non-consensus Clp binding sites are involved in upregulation of the gum operon involved in xanthan polysaccharide synthesis in Xanthomonas campestris pv. campestris.;Chen CH, Lin NT, Hsiao YM, Yang CY, Tseng YH;Research in microbiology 2010 Sep; 161(7):583-9 [20558281]

TF

Clp [Q4UZF6, view regulon]

Reported TF sp.

Xanthomonas campestris pv. campestris str.17

Reported site sp.

Xanthomonas campestris pv. campestris str.17

Created by

Dinara Sagitova

Curation notes

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Experimental Process

the transcriptional fusion assay results suggested that transcription of the gum promoter, while retaining a high basal level of expression without Clp, is upregulated by Clp. EMSAs using different gum promoter regions as probes showed that Clp binds specifically to the two tandem Clp binding sites of the gum promoter.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.