Curation Information

Publication: The PerR regulon in peroxide resistance and virulence of Streptococcus pyogenes.;Brenot A, King KY, Caparon MG;Molecular microbiology 2005 Jan; 55(1):221-34 [15612930]
TF: PerR [Q9A1M6, view regulon]
Reported TF sp.: Streptococcus pyogenes M1 GAS
Reported site sp.: Streptococcus pyogenes M1 GAS
Created by: Dinara Sagitova
Curation notes: -

Experimental Process

PerR binding site was identified by searching mrgA promoter region for a sequence similar to an experimentally confirmed PerR box. Quantitative real-time PCR analysis confirmed that PerR is a transcriptional repressor of mrgA.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

CGATAATGATTCTAA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
CGATAATGATTCTAA	dpr,		Experimental technique details qPCR [quantitative real-time] (ECO:0005660) qPCR [quantitative real-time] - ECO:0005660 Quantitative PCR (or quantitative real time polymerase chain reaction) is a modification of the conventional PCR reaction used to amplify and simultaneously quantify a targeted DNA molecule. In its more standard incarnation, a inactive fluorescent dye is added to the PCR mix. This dye is activated upon binding double-stranded DNA; as the PCR iterates, there is more and more dsDNA and therefore more fluorescence intensity. -	repressor	not specified