Curation Information

Publication: Regulation of the pcaIJ genes for aromatic acid degradation in Pseudomonas putida.;Parales RE, Harwood CS;Journal of bacteriology 1993 Sep; 175(18):5829-38 [8376330]
TF: PcaR [Q88N41, view regulon]
Reported TF sp.: Pseudomonas putida PRS2000
Reported site sp.: Pseudomonas putida PRS2000
Created by: Erill Lab
Curation notes: -

Experimental Process

Induction demonstrated with beta-gal assay with multiple fragments. Deletion of central site element GATAAT shown to be necessary for induction.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

GTTCGATAATCGCAC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
GTTCGATAATCGCAC	pcaI, pcaJ,		Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	repressor	not specified