Curation Information

Publication

SaPI operon I is required for SaPI packaging and is controlled by LexA.;Ubeda C, Maiques E, Tormo MA, Campoy S, Lasa I, Barbé J, Novick RP, Penadés JR;Molecular microbiology 2007 Jul; 65(1):41-50 [17581119]

TF

LexA [Q9L4P1, view regulon]

Reported TF sp.

Staphylococcus aureus strain RN3984

Reported site sp.

Staphylococcus aureus strain RN3984

Created by

Erill Lab

Curation notes

Staphylococcus aureus strain RN3984 mobile pathogenicity island SaPI2 (GenBank: EF010993.1) is not yet in RefSeq. Record needs to be updated and submitted when RefSeq record is introduced.

Experimental Process

Effect of LexA evidenced with LexA-ind mutant and rate of transduction of PAI. Sites identified through PSSM search with B. subtilis profile and MSA with other SaPIbov sequences. Binding of LexA verified by EMSA with multiple fragments and functionality of sites assessed by RT-PCR with mit-C induction on w-t and LexA-ind.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.