Curation Information

Publication

Analysis of ToxR-dependent transcription activation of ompU, the gene encoding a major envelope protein in Vibrio cholerae.;Crawford JA, Kaper JB, DiRita VJ;Molecular microbiology 1998 Jul; 29(1):235-46 [9701817]

TF

ToxR [A0A0H3AK48, view regulon]

Reported TF sp.

Vibrio cholerae O395

Reported site sp.

Vibrio cholerae O395

Created by

Elliot White

Curation notes

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Experimental Process

Sites were identified by fusing different length promoter fragments with a promoterless LacZ and comparing B-gal activity. The results of these assays suggest the presence of 3 sites in the ompU promoter. The existance of these sites was verified with emsas of the promoter fragments, and a DNase protection assay, which showed three footprints. The authors do not attempt to narrow down the sites further.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.