Curation Information

Publication: Identification, cloning, and sequencing of a gene required for ferric vibriobactin utilization by Vibrio cholerae.;Butterton JR, Calderwood SB;Journal of bacteriology 1994 Sep; 176(18):5631-8 [8083157]
TF: Fur [A5F6G4, view regulon]
Reported TF sp.: Vibrio cholerae O395
Reported site sp.: Vibrio cholerae O395
Created by: Erill Lab
Curation notes: -

Experimental Process

Northern blot shows iron-dependent expression of viuB. Visual inspection indicates site similar to E. coli consensus.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

GTTAATGATATACATTCTC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
GTTAATGATATACATTCTC	viuB,		Experimental technique details Northern blot (ECO:0005653) Northern blot - ECO:0005653 The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. Northern blotting involves the use of electrophoresis to separate RNA samples by size and detection with a hybridization probe complementary to part of or the entire target sequence. - Experimental technique details Visual sequence inspection (nan) Visual sequence inspection - nan Visual inspection of promoter region to identify putative binding sites based on previous knowledge -	repressor	not specified