Curation Information

Publication

Metal ion regulation of gene expression. Fur repressor-operator interaction at the promoter region of the aerobactin system of pColV-K30.;de Lorenzo V, Giovannini F, Herrero M, Neilands JB;Journal of molecular biology 1988 Oct 20; 203(4):875-84 [3062182]

TF

Fur [P0A9A9, view regulon]

Reported TF sp.

ColV-K30

Reported site sp.

ColV-K30

Created by

Urooq Aqeel

Curation notes

Sites belong to plasmid ColV-K30, which is not available in NCBI.

Experimental Process

Site directed mutagenesis and DNAse I nicking were used to find the fur binding site. Expression of fur was experimentally tested by hydroxyl radical foot-printing and methylation protection experiments.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.