Curation Information

Publication: Cloning and characterization of the Actinobacillus pleuropneumoniae fur gene and its role in regulation of ApxI and AfuABC expression.;Hsu YM, Chin N, Chang CF, Chang YF;DNA sequence : the journal of DNA sequencing and mapping 2003 Jun; 14(3):169-81 [14509829]
TF: Fur [A3N1M0, view regulon]
Reported TF sp.: Actinobacillus pleuropneumoniae App4074
Reported site sp.: Actinobacillus pleuropneumoniae App4074
Created by: Urooq Aqeel
Curation notes: -

Experimental Process

Fur regulation shown by luciferase assays. Site identified by visual inspection.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

GTAAATTATATAAATAATC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
GTAAATTATATAAATAATC	apxIC, apxIA,		Experimental technique details Luciferase reporter assay (ECO:0005648) Luciferase reporter assay - ECO:0005648 A type of reporter assay . The idea is identical to a betagalactosidase assay, but here fluorescence of luciferase gene product is used as the reporter. - Experimental technique details Visual sequence inspection (nan) Visual sequence inspection - nan Visual inspection of promoter region to identify putative binding sites based on previous knowledge -	activator	dimer