Curation Information

Publication
Characterization of the Pseudomonas aeruginosa transcriptional response to phenylalanine and tyrosine.;Palmer GC, Palmer KL, Jorth PA, Whiteley M;Journal of bacteriology 2010 Jun; 192(11):2722-8 [20304990]
TF
PhhR [A0A0C7D413, view regulon]
Reported TF sp.
Pseudomonas aeruginosa UCBPP-PA14
Reported site sp.
Pseudomonas aeruginosa UCBPP-PA14
Created by
Kathryn Cronise
Curation notes
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Experimental Process

A DNA microarray was used to confirm the role of PhhR in the expression of phhA, hpd, and dhcA. A consensus search followed by EMSA was used to prove binding.

Transcription Factor Binding Sites


CGTCAAGAATATGTGACA
CGTAAGGAAAACTTTACG
TGTAAAGATAATTTTACG
GGAAAAACAAACTTAACA
CGTCAAGAATATGTGACA
CGTAAGGAAAACTTTACG
TGTAAAGATAATTTTACG
GGAAAAACAAACTTAACA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CGTCAAGAATATGTGACA phhA,
... ... phhA phhR
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - activator not specified
CGTAAGGAAAACTTTACG phhA,
... ... phhA phhR
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - activator not specified
TGTAAAGATAATTTTACG hpd,
... ... hpd PA14_53090 PA14_53100 PA14_53110
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - activator not specified
GGAAAAACAAACTTAACA PA14_38660,
... ... PA14_38660 PA14_38640 PA14_38680
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - activator not specified