A DNA microarray was used to confirm the role of PhhR in the expression of phhA, hpd, and dhcA. A consensus search followed by EMSA was used to prove binding.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
Site sequence | Regulated genes | Gene diagram | Experimental techniques | TF function | TF type |
---|---|---|---|---|---|
CGTCAAGAATATGTGACA | phhA, |
|
activator | not specified | |
CGTAAGGAAAACTTTACG | phhA, |
|
activator | not specified | |
TGTAAAGATAATTTTACG | hpd, |
|
activator | not specified | |
GGAAAAACAAACTTAACA | PA14_38660, |
|
activator | not specified |