Curation Information

Publication: Deletion of the ferric uptake regulator Fur impairs the in vitro growth and virulence of Actinobacillus pleuropneumoniae.;Jacobsen I, Gerstenberger J, Gruber AD, Bossé JT, Langford PR, Hennig-Pauka I, Meens J, Gerlach GF;Infection and immunity 2005 Jun; 73(6):3740-4 [15908404]
TF: Fur [B3H1Z1, view regulon]
Reported TF sp.: Actinobacillus pleuropneumoniae serovar 1 str. 4074
Reported site sp.: Actinobacillus pleuropneumoniae serovar 1 str. 4074
Created by: Urooq Aqeel
Curation notes: -

Experimental Process

Evidence of Fur regulation through Western blots with fur mutants, iron induction and w-t. Transcriptional start-point for exbBD-tbpBA determined by RACE-PCR. High-quality Fur box observed to overlap with TSS.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

GATAATGATTTTCATTAAC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
GATAATGATTTTCATTAAC	APP7_1631, APP7_1629,		Experimental technique details Visual sequence inspection (nan) Visual sequence inspection - nan Visual inspection of promoter region to identify putative binding sites based on previous knowledge - Experimental technique details Western blot (quantitative) expression analysis (ECO:0000279) Western blot (quantitative) expression analysis - ECO:0000279 A Western blot (also frequently called immunoblot) is a gel-based technique used to determine the presence of specific proteins in a sample by means of separation (through gel electrophoresis) and detection (using a protein-specific antibody). Less frequent than the equivalent RNA Northern blot, it is typically used in TF-binding site analysis to validate differential regulation of target genes. -	activator	dimer