Curation Information

Publication: Transcriptional regulation of Pseudomonas aeruginosa rhlR: role of the CRP orthologue Vfr (virulence factor regulator) and quorum-sensing regulators LasR and RhlR.;Croda-García G, Grosso-Becerra V, Gonzalez-Valdez A, Servín-González L, Soberón-Chávez G;Microbiology (Reading, England) 2011 Sep; 157(Pt 9):2545-55 [21719541]
TF: Vfr [P55222, view regulon]
Reported TF sp.: Pseudomonas aeruginosa PAO1
Reported site sp.: Pseudomonas aeruginosa PAO1
Created by: Kathryn Cronise
Curation notes: -

Experimental Process

A beta-gal assay showed that Vfr is involved in rhlR expression. A consensus search identified 4 binding sites. Each site was knocked out and a beta-gal assay was performed to see the effect. One repression site was identified.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

ATTGTCACAACCGCACAG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
ATTGTCACAACCGCACAG	rhlR,		Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. - Experimental technique details Consensus search (ECO:0005624) Consensus search - ECO:0005624 This is a weak form of in-silico search, in which the consensus sequence for the motif is compared to genomic positions and the number of mismatches (between candidate site and consensus) is used as a measure of site-quality. -	repressor	not specified