Curation Information

Publication

Transcriptional regulatory cascade for elastase production in Vibrio vulnificus: LuxO activates luxT expression and LuxT represses smcR expression.;Roh JB, Lee MA, Lee HJ, Kim SM, Cho Y, Kim YJ, Seok YJ, Park SJ, Lee KH;The Journal of biological chemistry 2006 Nov 17; 281(46):34775-84 [16971386]

TF

LuxT [Q7MFA2, view regulon]

Reported TF sp.

Vibrio vulnificus MO6-24/O

Reported site sp.

Vibrio vulnificus MO6-24/O

Created by

Elliot White

Curation notes

Multiple variations of the DNase protected site were tried, including several truncations; none returned the correct site. The further site mentioned in the paper has no binding data associated with it.

Experimental Process

Used site-directed mutagenesis to recreate the LuxT putative lux box in the upstream region of the pGEM plasmid, regulating luciferase. Used western blot analysis to compare protein output of wt vs ∆luxT. ∆luxT strain was generated using a deletion. Binding of LuxT was determined first by EMSA, then narrowed down via DNase Footprinting

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.