Curation Information

Publication: The Staphylococcus aureus CsoR regulates both chromosomal and plasmid-encoded copper resistance mechanisms.;Baker J, Sengupta M, Jayaswal RK, Morrissey JA;Environmental microbiology 2011 Sep; 13(9):2495-507 [21812885]
TF: CsoR [A0A0H3K9R5, view regulon]
Reported TF sp.: Staphylococcus aureus subsp. aureus str. Newman
Reported site sp.: Staphylococcus aureus subsp. aureus str. Newman
Created by: Erill Lab
Curation notes: -

Experimental Process

A CsoR homologue in S. aureus was identified by computational search (36% identical to the B. subtilis CsoR). Total RNA was extracted from wild-type and CsoR mutants and analyzed by Northen blot to show that CsoR is responsible for copper-dependent repression of copAZ and copBmco operons, and their operon configuration confirmed. The expression of other genes under low and high copper conditions in the w-t and CsoR mutant was investigated by RT-PCR, revealing repression of copAZ and clpC.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

ATACCTATAGGGGGTAC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
ATACCTATAGGGGGTAC	NWMN_2457		Experimental technique details Northern blot (ECO:0005653) Northern blot - ECO:0005653 The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. Northern blotting involves the use of electrophoresis to separate RNA samples by size and detection with a hybridization probe complementary to part of or the entire target sequence. - Experimental technique details qRT-PCR [RNA] (ECO:0001808) qRT-PCR [RNA] - ECO:0001808 Quantitative Reverse-Transcription PCR is a modification of PCR in which RNA is first reverse transcribed into cDNA and this is amplified measuring the product (qPCR) in real time. It therefore allows one to analyze transcription by directly measuring the product (RNA) of a gene's transcription. If the gene is transcribed more, the starting product for PCR is larger and the corresponding volume of amplification is also larger. - Experimental technique details Visual sequence inspection (nan) Visual sequence inspection - nan Visual inspection of promoter region to identify putative binding sites based on previous knowledge -	repressor	not specified