Curation Information

Publication

Lrp binds to two regions in the dadAX promoter region of Escherichia coli to repress and activate transcription directly.;Zhi J, Mathew E, Freundlich M;Molecular microbiology 1999 Apr; 32(1):29-40 [10216857]

TF

Lrp [P0ACJ0, view regulon]

Reported TF sp.

Escherichia coli str. K-12 substr. MG1655

Reported site sp.

Escherichia coli str. K-12 substr. MG1655

Created by

Erill Lab

Curation notes

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Experimental Process

MPE·Fe(II) footprinting was used to precisely identify binding sites for Lrp in the dad promoter. LacZ reporter assays with mutagenized sites were then used to verify their activity in the presence or absence of alanine.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.