Curation Information

Publication

Transcriptional regulation of sdiA by cAMP-receptor protein, LeuO, and environmental signals in Salmonella enterica serovar Typhimurium.;Turnbull AL, Kim W, Surette MG;Canadian journal of microbiology 2012 Jan; 58(1):10-22 [22149171]

TF

LeuO [P46924, view regulon]

Reported TF sp.

Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S

Reported site sp.

Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S

Created by

Dinara Sagitova

Curation notes

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Experimental Process

sdiA expression was shown to be reduced by 30% in leuO deletion mutant compared to the wild-type strain. EMSA confirmed that LeuO regulated sdiA expression directly. Footprinting further confirmed the EMSA results by showing that LeuO bound to two sites which overlap with the -35 and -10 sequences in the sdiA promoter.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.